GHRU Protocols

GHRU Protocols

The links below are to the latest copies of the protocols, SOPs and training materials

Whole Genome Sequencing (Wet-lab)

Protocols

Methods and workflows as developed for "Empowering microbiology laboratories in emerging economies with scalable genome sequencing for routine pathogen surveillance" CID manuscript

Full List of wet-lab SOPs

1. Genomic DNA Isolation

   Bacterial Genomic DNA Isolation using the Nexttec 1-step Kit

   Genomic DNA Quantification using a Qubit Fluorometer

   Genomic DNA Purity Measurement using a Nanodrop Spectrophotometer

   
   

2. DNA Library Preparation

   DNA Library Preparation using the NEBNext Ultra II FS DNA Kit for Illumina

   Quality Control (QC) of DNA Libraries for Whole Genome Sequencing

   
   

3. Library Normalisation and Pooling

   Normalisation and Pooling of DNA Libraries for Illumina Whole Genome Sequencing

   
   

4. Whole Genome Sequencing

   Operation of the Illumina MiSeq for Whole Genome Sequencing

   
   

5. DNA Sample Transport

   Preparation of DNA Isolates for Domestic and Overseas Transport

Bioinformatics

Protocols

Methods used for the "Overcoming data bottlenecks in pathogen genomic surveillance" CID manuscript

Training Materials

Bioinformatics Training